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Flow Cytometer

BD FACSAria Cell Sorting System( Flow cytometer)

The BD FACSAria instrument is the first benchtop flowcytometer with sorter that incorporates a fixed-alignment cuvette flow cell. This new flow cell provides superior fluorescence sensitivity. The fixed optical system offers freedom from instrument maintenance and alignment. It accommodates two air-cooled lasers at 488-nm, 633-nm and analyse 9 parameters simultaneousely. It can achieve Digital acquisition rates of up to 70,000 events/second. At present we are performing following tests on this machine:

  • Leukemia immunophenotyping
  • Diagnosis of Paroxysmal Haemoglobinuria
  • Analysis of major and minor lymphocyte subsets
  • Diagnosis of platelet function defects
  • Diagnosis of different primary immunodeficiency disorders
  • Diagnosis of red cell membrane defects
  • Characterization of haematopoietic and mesenchymal stem cells
  • Sorting of fetal NRBCs from maternal blood
  • Sorting of lymphocytes and neutrophils
  • Sorting of CD34 positive cells