आई सी एम आर - राष्ट्रीय प्रतिरक्षा रुधिर विज्ञान संस्थान

ICMR-National Institute of Immunohaematology

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Tests performed by the Department of Pediatric Immunology & Leukocyte Biology
Lymphocyte subset analysis:
T cells and their subsets:
  • T helper (CD4+)/ T cytotoxic (CD8+)
  • Naïve (CCR7+/CD62L+/CD27+/CD45RA+)
  • Memory (CCR7+/CD62L+/CD27+/CD45RA-)
  • Effector (CCR7-/CD62L-/CD27-/CD45RA-)
  • TEMRA (CCR7+/CD62L+/CD27+/CD45RA-)
  • TsCM (CCR7+CD45RA+CD28+CD95+)
  • CD8+CD28-Tcell
  • Senescent (CD57+CD28-)
  • Exhausted (PD1+/Tim3+)
  • T follicular helper (CXCR5+CD45RA-PD1+)
  • Th1/ Tc1 like (CXCR3+CCR4-)
  • Th2/Tc2 like (CXCR3-CCR4+CCR6-)
  • Th17/ Tc17like (CCR4+CCR6+CCR10-)
  • Th9(CCR4-CCR6+)
  • Th22 (CCR4+CCR10+CCR6+)
  • Recent thymic emigrants (CD31+CD62L+CD45RA+)
  • ab + T cell (GD-)
  • gd + T cell (GD+)
Regulatory T cells and their subsets:
  • Treg (CD4/CD8) (CD25++CD127-/low)
  • Memory
  • Treg (CD95+CCR4+CD45RAlowHLADR+)
  • Naïve Treg (CD45RA+CCR4-/lowCD95-)
  • V beta repertoire analysis
B cells and their subsets:
  • Naïve (CD27-IgD+)
  • Memory (CD27+IgD+)
  • Class switched memory (CD27-IgM-IgD-)
  • Germinal centre (CD27+IgD-)
  • Marginal zone (CD27+IgD+IgM+)
  • IgM only memory (CD27+IgD-IgM+)
  • CD 21 low (CD21-/CD38-)
  • Plasmablast (CD27+CD24+CD38+)
  • Activated B cell (CD95+CD38-)
  • B reg (CD24++CD38++)
  • Transitional 1 (CD27-IgD+ CD24+ CD10+ CD38+)
  • Transitional 2 (CD27-IgD+CD10-CD38+)
  • Transitional 3 (CD27-IgD+CD10-CD38-)
DNT cells and their subsets:
  • B220+ : proliferative DNTs
  • CD27+CD28+ : Naïve DNTs
  • CD45RA-CD27-: Effectors DNTs
  • CD57+KLRG1- : senescent DNTs
Surface protein expression for the diagnosis
of specific IEIS:
  • Btk: X-linked agammaglobulinemia
  • CD127: IL-7 receptor a deficiency
  • CD132: X-linked SCID
  • CD18/CD11a/CD11b /CD11c: LAD-I
  • CD40: AR Hyper IgM syndrome
  • CD40L: X-linked HyperIgM syndrome
  • DOCK 8: DOCK-8 deficiency
  • WASp: Wiskott Aldrich Syndrome
  • p47, p22, p67: Chronic Granulomatous Disease
  • IL12Rb1: MSMD
  • IFNgR1: MSMD
  • CTLA4: CTLA4 haploinsufficiency
  • LRBA: LRBA deficiency
  • Perforin: FHL2
  • XIAP: XLP2
  • FoxP3: IPEX syndrome
  • NKg2D expression : XMEN syndrome
Functional Assays:
  • pSTAT1, pSTAT3, pSTAT4, pSTAT5
  • Granule release assay
  • NBTDHR/FMLP.
  • Apoptosis assay
  • CD154 expression
  • CD62L shedding
  • RBC ADA levels
  • T cell proliferation by PHA and anti CD3 and CD28
Other specialized tests:
  • TREC
  • T cell- V beta repertoire
  • Immunoglobulin levels: IgG, IgA, IgM, IgE levels
  • IgG subclass analysis (IgG2 and IgG4) and specific antibody responses (anti-tetanus antibodies and anti-typhoid antibodies)
  • Complement system evaluation: CH50 and AH50
Tests performed by the Department of Tests by Cytogenetics
  • Karyotyping from Blood and Bone marrow
  • Chromosomal breakage investigation for Fanconi anemia
  • Fluorescence in situ hybridisation (FISH) for Chronic myeloid leukaemia, Myelodysplastic syndrome and other genetic diseases
  • RT-PCR, Tyrosine kinase domain mutations (TKD) in CML
  • Molecular diagnosis of Fanconi?s anemia and other Inherited Bone marrow failure syndromes (IBMFS)
  • Prenatal diagnosis of Fanconi anemia
Tests performed by the Department of Clinical & Experimental Immunology
  • Antinuclear Antibody (IFA) test
  • Anti -double stranded DNA antibody (Anti- ds DNA) (IFA) test
  • Anti-neutrophil cytoplasmic Antibody (ANCA) (IFA) test
  • ANA profile 3(IgG) (ANA Blot) Immunoblot Test
  • Anti-MPO, -PR3, and anti-GBM(IgG) (ANCA Blot) Immunoblot Test
  • Anti-Desmoglein 1(Dsg1) antibody (IgG) (DSG1)
  • Anti- Desmoglein 3 (Dsg3) antibody (IgG) (DSG3)
  • Anti-BP180 antibody(IgG) (BP180)
  • Anti-BP230 antibody (IgG) (BP-230)
Tests performed by the Department of Haematogenetics
  • Detection of Haemoglobinopathies
  • Alpha Thai Workup
  • Beta Thai Workup
  • Prenatal Diagnosis of Hemoglobinopathies (Family)
  • Red Cell Enzyme Assay
  • Red Cell Membrane Study
  • Methemoglobin Workup
  • Prenatal Diagnosis for rare Enzymopathy, membranopat hy and recessive congenital methemoglobinemia
  • G6PD Screening
  • Congenital Dyserythropeotic Anemia Workup
  • Unstable Hb Workup
  • KB Staining
  • Alpha triplication study
  • Gilbert Syndrome
  • Unstable Hb
  • Alpa & Beta Thal Workup
  • RBC Membarance & Enzyme Study
Tests performed by the Department of Haemostasis and Thrombosis
  • Screening Coagulation- Prothrombin Time (PT) Activated Partial Thromboplastin Time (aPTT) Thrombin Time (TT)
  • Factor Inhibitor Screening
  • Nijmegen Modified Bethesda Assay (NBA)
  • Common Factor Assays: FVIII/FIX
  • Rare Factor Assays: F II,FV,FVII,FX,FXI,FXII
  • Platelet Receptors: GP IIBIIIA(CD41a),GP IX(CD42a) , GP IB(CD42b) ,Fibrinogen(FIB), GP IIB(CD41),GPIIIA(CD61)
  • Platelet Aggregation :ADP, RISTOCETIN(RISTO), COLLAGEN(COL), ARACHIDONIC ACID(A.A.)
  • Von Willebrand factor (VWF)Antigen Rocket
  • Von Willebrand factor (VWF)Antigen Automated
  • Ristocetin Cofactor assay (RiCof)
  • Factor XIII Urea Clot Lysis
  • Fibrinogen
  • Thrombophilia : Free protein S Level ,Protein C Activity, Antithrombin Activity, Factor V Leiden Mutation
  • ADAMTS 13 activity
  • ADAMTS 13 AUTOANTIBODY
  • Human Complement Factor H assay(CFH)
  • Anti- Complement Factor H assay(Anti-CFH)
  • Human Complement Factor I assay(CFI)
  • Alpha 2 Antiplasmin
  • Carrier Diagnosis Of Haemophilia And Other Bleeding Disorders: Known Mutations (by direct sequencing)
  • Carrier Diagnosis Of Haemophilia And Other Bleeding Disorders: Unknown Mutations (by direct sequencing)
  • Antenatal Diagnosis For Haemophilia and Other bleeding disorders: Known Mutations (by direct sequencing)
  • Antenatal Diagnosis For Haemophilia and Other bleeding disorders: Unknown Mutations (by direct sequencing)
  • Human Platelet Antigen (HPA) Genotyping
Tests performed by the Department of Transfusion Medicine
  • Direct and Indirect Antiglobulin test (DAT/IAT)
  • Blood group confirmation
    a. Bombay blood group
    b. ABO weaker variant
    c. RhD variant
  • ABO antibody Titre
  • RhD antibody Titre
  • Rh Zygosity
  • Antibody study
  • Autoimmne Hemolytic Anemia whole workup (Blood grouping, DAT, IAT, Genotyping, Eution of antibody, Antibody identification)
  • Extended antigen profile by molecular assay
Tests performed by the Department of Transfusion Transmitted Diseases
  • HBeAg (Hepatitis B `e` antigen)
  • Anti HBs titre (Hepatitis B surface antibody titre)
  • Anti-HBc IgM/IgG (Hepatitis B core antibody IgM/IgG)
  • HBV DNA/ HCV RNA (Hepatitis B/ Hepatitis C viral load)
  • HCV genotyping (Hepatitis C virus genotyping)
  • Family screening for HBsAg and anti-HBc
  • HBV profile (HBeAg + HBV DNA)

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